fetal human brain tissue were subjected to SDS PAGE followed by western blot with FNab09217(UCHL1 Antibody) at dilution of 1:20000
Immunohistochemistry of paraffin-embedded human hypothalamus tissue slide using FNab09217(UCHL1 Antibody) at dilution of 1:50
Immunofluorescent analysis of Neuro-2a cells using FNab09217(UCHL1 Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-mouse IgG(H+L)
Background
Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.